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fibroblast growth medium  (PromoCell)


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    Structured Review

    PromoCell fibroblast growth medium
    Schematic overview of the workflow developed in this study. Human iPSCs were differentiated into cardiomyocytes (CMs), endothelial cells (ECs), and cardiac <t>fibroblasts</t> (CFs) using serum-free protocols, wherein fetal bovine serum was replaced by Panexin for stromal lineages. For engineered heart tissue (EHT) fabrication, 10% v/v human serum efficiently supported morphological remodelling and functional contractility in CM-only EHTs. Triculture EHTs assembled from CMs (70%), ECs (15%), and CFs (15%) showed enhanced structural organisation and contractile performance under high (10% v/v) and low (1% v/v) human serum conditions in the absence of prolonged TGFβ inhibition by SB431542. Representative contraction traces illustrate functional differences between monoculture and triculture EHTs under optimised conditions.
    Fibroblast Growth Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibroblast+growth+medium+3+kit/Fibroblast+Growth+Medium+3+Kit/bio_rxiv__64898__2026__01__28__702392-111-12-16
    Average 94 stars, based on 26 article reviews
    fibroblast growth medium - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Efficient multi-lineage cardiovascular differentiation of human pluripotent stem cells in animal serum-free conditions"

    Article Title: Efficient multi-lineage cardiovascular differentiation of human pluripotent stem cells in animal serum-free conditions

    Journal: bioRxiv

    doi: 10.64898/2026.01.28.702392

    Schematic overview of the workflow developed in this study. Human iPSCs were differentiated into cardiomyocytes (CMs), endothelial cells (ECs), and cardiac fibroblasts (CFs) using serum-free protocols, wherein fetal bovine serum was replaced by Panexin for stromal lineages. For engineered heart tissue (EHT) fabrication, 10% v/v human serum efficiently supported morphological remodelling and functional contractility in CM-only EHTs. Triculture EHTs assembled from CMs (70%), ECs (15%), and CFs (15%) showed enhanced structural organisation and contractile performance under high (10% v/v) and low (1% v/v) human serum conditions in the absence of prolonged TGFβ inhibition by SB431542. Representative contraction traces illustrate functional differences between monoculture and triculture EHTs under optimised conditions.
    Figure Legend Snippet: Schematic overview of the workflow developed in this study. Human iPSCs were differentiated into cardiomyocytes (CMs), endothelial cells (ECs), and cardiac fibroblasts (CFs) using serum-free protocols, wherein fetal bovine serum was replaced by Panexin for stromal lineages. For engineered heart tissue (EHT) fabrication, 10% v/v human serum efficiently supported morphological remodelling and functional contractility in CM-only EHTs. Triculture EHTs assembled from CMs (70%), ECs (15%), and CFs (15%) showed enhanced structural organisation and contractile performance under high (10% v/v) and low (1% v/v) human serum conditions in the absence of prolonged TGFβ inhibition by SB431542. Representative contraction traces illustrate functional differences between monoculture and triculture EHTs under optimised conditions.

    Techniques Used: Functional Assay, Inhibition

    Related Articles

    Cell Culture:

    Article Title: Unsupervised inter-domain transformation for virtually stained high-resolution mid-infrared photoacoustic microscopy using explainable deep learning
    Article Snippet: Stage-raster scanning was driven by two motorized linear stages (L-406.10SD00, Physik Instrumente), and the laser fluence, detected by a mercury cadmium telluride amplified photodetector (PDAVJ10, Thorlabs), was used to calibrate the PA signal pixel-by-pixel. .. HCFs (C-12375, Promocell) up to passage 6 were cultured in a Fibroblast Growth Medium 3 kit (C-23130, Promocell) supplemented with 1% penicillin-streptomycin (SV30010, Hyclone). ..

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The hiPSC line, 1390D4 (CiRA), was cultured in StemFit®AK02N (Ajinomoto) on iMatrix-511 (Nippi)-coated dishes. .. Human cardiac fibroblasts (HCFs) (Promo Cell) were cultured in a Fibroblast Growth Medium 3 kit (Promo Cell). .. The cardiomyocyte differentiation method using embryoid bodies (EBs) was previously described.3 For cardiomyocyte purification, day 10–15 EBs were cultured in DMEM without glucose, L-glutamine, and phenol red (Thermo Fisher) supplemented with 4 mM L-lactic acid (Wako) and 1 mM sodium pyruvate (Thermo Fisher Science) for 3 days.

    Article Title: diomyopathy phenotypes of engineered cardiac tissue through maturation
    Article Snippet: Normal Human Dermal Fibroblasts, Adult (NHDFs) (female) were purchased from Lonza and cultured in high glucose Dulbecco’s modified Eagle's medium supplemented with 10% FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin or Fibroblast Growth Medium-2 BulletKitTM (Lonza) at 37°C in 5% CO2 according to manufacturer’s protocol. .. Human cardiac fibroblast were purchased PromoCell and cultured in Fibroblast Growth Medium 3 Kit (PromoCell). .. For cardiomyocyte differentiation using embryoid bodies (EBs), single hiPSCs were seeded at 2 × 106 cells/well in a StemPro-34-based medium composed of StemPro-34 SFM (Thermo Fisher Science), 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg/mL ascorbic acid (Sigma), and 150 μg/mL Transferrin (Wako), supplemented with 10 μM Y-27632(Wako), 2 ng/mL BMP-4 (R&D Systems), and 0.5% Matrigel (Corning), onto poly(2-hydroxyethyl methacrylate)-coated 6-well plate.

    Article Title: ERRg agonist under mechanical stretching manifests hypertrophic cardiomyopathy phenotypes of engineered cardiac tissue through maturation
    Article Snippet: Normal Human Dermal Fibroblasts, Adult (NHDFs) (female) were purchased from Lonza and cultured in high glucose Dulbecco’s modified Eagle's medium supplemented with 10% FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin or Fibroblast Growth Medium-2 BulletKitTM (Lonza) at 37°C in 5% CO2 according to manufacturer’s protocol. .. Human cardiac fibroblast were purchased PromoCell and cultured in Fibroblast Growth Medium 3 Kit (PromoCell). .. For cardiomyocyte differentiation using embryoid bodies (EBs), single hiPSCs were seeded at 2 × 106 cells/well in a StemPro-34-based medium composed of StemPro-34 SFM (Thermo Fisher Science), 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg/mL ascorbic acid (Sigma), and 150 μg/mL Transferrin (Wako), supplemented with 10 μM Y-27632(Wako), 2 ng/mL BMP-4 (R&D Systems), and 0.5% Matrigel (Corning), onto poly(2-hydroxyethyl methacrylate)-coated 6-well plate.



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    Image Search Results


    Schematic overview of the workflow developed in this study. Human iPSCs were differentiated into cardiomyocytes (CMs), endothelial cells (ECs), and cardiac fibroblasts (CFs) using serum-free protocols, wherein fetal bovine serum was replaced by Panexin for stromal lineages. For engineered heart tissue (EHT) fabrication, 10% v/v human serum efficiently supported morphological remodelling and functional contractility in CM-only EHTs. Triculture EHTs assembled from CMs (70%), ECs (15%), and CFs (15%) showed enhanced structural organisation and contractile performance under high (10% v/v) and low (1% v/v) human serum conditions in the absence of prolonged TGFβ inhibition by SB431542. Representative contraction traces illustrate functional differences between monoculture and triculture EHTs under optimised conditions.

    Journal: bioRxiv

    Article Title: Efficient multi-lineage cardiovascular differentiation of human pluripotent stem cells in animal serum-free conditions

    doi: 10.64898/2026.01.28.702392

    Figure Lengend Snippet: Schematic overview of the workflow developed in this study. Human iPSCs were differentiated into cardiomyocytes (CMs), endothelial cells (ECs), and cardiac fibroblasts (CFs) using serum-free protocols, wherein fetal bovine serum was replaced by Panexin for stromal lineages. For engineered heart tissue (EHT) fabrication, 10% v/v human serum efficiently supported morphological remodelling and functional contractility in CM-only EHTs. Triculture EHTs assembled from CMs (70%), ECs (15%), and CFs (15%) showed enhanced structural organisation and contractile performance under high (10% v/v) and low (1% v/v) human serum conditions in the absence of prolonged TGFβ inhibition by SB431542. Representative contraction traces illustrate functional differences between monoculture and triculture EHTs under optimised conditions.

    Article Snippet: Following WNT activation and inhibition phases, cells were replated and matured in fibroblast growth medium (FGM, PromoCell #C-23130) supplemented with Panexin (replacing fetal calf serum), 10 ng/ml FGF2 and 10 μM SB431542, where indicated.

    Techniques: Functional Assay, Inhibition

    Figure 1. Development of vascularized EHT module. a) Schematic diagram illustrating the EHT modules and their cell compositions (CM, cardiomyo- cyte; cFB, cardiac fibroblast; EC, endothelial cell). b) Fluorescence images showing harmonious integration of encapsulated cells within tissue as time progressed (green, DiO-labeled CMs and cFBs; red, Red fluorescent protein-tagged HUVECs; scale bar, 500 μm). c) LIVE/DEAD assessment showing cell viability within the EHT modules at days 1 and 14 after tissue fabrication (green, Calcein-AM; red, ethidium homodimer-1; scale bar, 500 μm), and the quantification of signal-positive areas indicating the cell viability (n = 4, biological replicates).

    Journal: Advanced NanoBiomed Research

    Article Title: Bioprinting‐Assisted Tissue Assembly to Investigate Endothelial Cell Contributions in Cardiac Fibrosis and Focal Fibrosis Modeling

    doi: 10.1002/anbr.202400148

    Figure Lengend Snippet: Figure 1. Development of vascularized EHT module. a) Schematic diagram illustrating the EHT modules and their cell compositions (CM, cardiomyo- cyte; cFB, cardiac fibroblast; EC, endothelial cell). b) Fluorescence images showing harmonious integration of encapsulated cells within tissue as time progressed (green, DiO-labeled CMs and cFBs; red, Red fluorescent protein-tagged HUVECs; scale bar, 500 μm). c) LIVE/DEAD assessment showing cell viability within the EHT modules at days 1 and 14 after tissue fabrication (green, Calcein-AM; red, ethidium homodimer-1; scale bar, 500 μm), and the quantification of signal-positive areas indicating the cell viability (n = 4, biological replicates).

    Article Snippet: HCFs were cultured in fibroblast growth medium kit (C-23130, Promocell) supplemented with 1% P/S.

    Techniques: Fluorescence, Labeling

    Figure 2. Vascular network formation leading to complex tissue formation and signaling pathway activation. a) IF image exhibiting the structure and distribution of each cell using cardiomyocyte-, fibroblast- and endothelial cell-specific markers within the tissue (green, α-sarcomeric actin; blue, VIM; red, CD31; scale bar, 100 μm). b) RNA sequencing heatmap showing 352 DEGs between CMF and CMFE. c) Bubble plot of KEGG pathway enrichment analysis of DEGs. The gene ratio indicates the ratio of enriched DEGs to background genes.

    Journal: Advanced NanoBiomed Research

    Article Title: Bioprinting‐Assisted Tissue Assembly to Investigate Endothelial Cell Contributions in Cardiac Fibrosis and Focal Fibrosis Modeling

    doi: 10.1002/anbr.202400148

    Figure Lengend Snippet: Figure 2. Vascular network formation leading to complex tissue formation and signaling pathway activation. a) IF image exhibiting the structure and distribution of each cell using cardiomyocyte-, fibroblast- and endothelial cell-specific markers within the tissue (green, α-sarcomeric actin; blue, VIM; red, CD31; scale bar, 100 μm). b) RNA sequencing heatmap showing 352 DEGs between CMF and CMFE. c) Bubble plot of KEGG pathway enrichment analysis of DEGs. The gene ratio indicates the ratio of enriched DEGs to background genes.

    Article Snippet: HCFs were cultured in fibroblast growth medium kit (C-23130, Promocell) supplemented with 1% P/S.

    Techniques: Activation Assay, RNA Sequencing